July 9, 2019

Nat Methods (2019) "Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM"

Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization micr...

January 1, 2018

Nat Chem (2018) "Chromophore twisting in the excited state of a photoswitchable fluorescent protein captures by time-resolved serial femtosecond crystallography"

Chromophores absorb light in photosensitive proteins and thereby initiate fundamental biological processes s...

December 1, 2017

In the frame of the "Ecole de Physique des Houches", a series of courses aimed at selected PhD students and young researchers will be given about fluorescent labels used in advanced fluorescence microscopy: "Fluorescence Markers for Advanced Microscopy: from Photophysi...

September 6, 2017

J Phys Chem Lett (2017) "Photoswitching of green mEos2 by intense 561 nm light perturbs efficient green-to-red photoconversion in localization microscopy"

Green-to-red photoconvertible fluorescent proteins (PCFPs) such as mEos2 and its derivatives are widely used in Pho...

February 25, 2016

J Phys Chem Lett (2016) "Serial femtosecond crystallography and ultrafast absorption spectroscopy of the photoswitchable fluorescent protein IrisFP"

Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in pa...

January 21, 2016

J Am Chem Soc (2016) "Arginine 66 controls dark-state formation in green-to-red photoconvertible fluorescent proteins"

Photoactivated localization microscopy (PALM) is a powerful technique to investigate cellular nanostructures quantitatively and dynamically. However, t...

January 21, 2016

Scientific Reports (2016) "Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm"

Phototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, th...

August 18, 2015

mBio (2015) "Remodeling of the Z-ring nanostructure during the Streptococcus pneumoniae cell cycle revealed by photoactivated localization microscopy"

Ovococci form a morphological group that includes several human pathogens (enterococci and streptococci). Their shape r...

January 16, 2015

Methods Appl. Fluoresc. (2015) "Rational design of enhanced photoresistance in a photoswitchable fluorescent protein"

Fluorescent proteins are particularly susceptible to photobleaching, the permanent loss of fluorescence emission resulting from photodestruction of the...

December 15, 2014

Photoniques (2014) "La microscopie de fluorescence à super-résolution : un outil révolutionnaire pour imager le vivant à l’échelle nanométrique"

Stefan Hell, Eric Betzig et William Moerner ont reçu le prix Nobel de chimie 2014 pour avoir développé un ensemble de techniq...

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