May 28, 2020

J Am Chem Soc (2020) "Mechanistic investigations of green mEos4b reveal a dynamic long-lived dark state"

Green-to-red photoconvertible fluorescent proteins (PCFPs) are key players in advanced microscopy schemes such as photoactivated localization microscopy (PALM). Wher...

February 7, 2020

Nat Comm (2020) "Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy"

Reversibly switchable fluorescent proteins (RSFPs) serve as markers in advanced fluorescence imaging. Photoswitching from...

February 25, 2016

J Phys Chem Lett (2016) "Serial femtosecond crystallography and ultrafast absorption spectroscopy of the photoswitchable fluorescent protein IrisFP"

Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in pa...

January 21, 2016

J Am Chem Soc (2016) "Arginine 66 controls dark-state formation in green-to-red photoconvertible fluorescent proteins"

Photoactivated localization microscopy (PALM) is a powerful technique to investigate cellular nanostructures quantitatively and dynamically. However, t...

January 21, 2016

Scientific Reports (2016) "Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm"

Phototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, th...

August 18, 2015

mBio (2015) "Remodeling of the Z-ring nanostructure during the Streptococcus pneumoniae cell cycle revealed by photoactivated localization microscopy"

Ovococci form a morphological group that includes several human pathogens (enterococci and streptococci). Their shape r...

January 16, 2015

Methods Appl. Fluoresc. (2015) "Rational design of enhanced photoresistance in a photoswitchable fluorescent protein"

Fluorescent proteins are particularly susceptible to photobleaching, the permanent loss of fluorescence emission resulting from photodestruction of the...

November 6, 2013

We deposited three new structures of IrisFP  bleached in anaerobic conditions, bleached by X-rays and bleached in aerobic conditions (PDB ID: 4LJB, 4LJC and 4LJD, respectively). These new structures demonstrate the two photobleaching pathways of fluorescent proteins we...

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