May 28, 2020

J Am Chem Soc (2020) "Mechanistic investigations of green mEos4b reveal a dynamic long-lived dark state"

Green-to-red photoconvertible fluorescent proteins (PCFPs) are key players in advanced microscopy schemes such as photoactivated localization microscopy (PALM). Wher...

February 7, 2020

Nat Comm (2020) "Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy"

Reversibly switchable fluorescent proteins (RSFPs) serve as markers in advanced fluorescence imaging. Photoswitching from...

January 1, 2018

Nat Chem (2018) "Chromophore twisting in the excited state of a photoswitchable fluorescent protein captures by time-resolved serial femtosecond crystallography"

Chromophores absorb light in photosensitive proteins and thereby initiate fundamental biological processes s...

November 6, 2013

We deposited three new structures of IrisFP  bleached in anaerobic conditions, bleached by X-rays and bleached in aerobic conditions (PDB ID: 4LJB, 4LJC and 4LJD, respectively). These new structures demonstrate the two photobleaching pathways of fluorescent proteins we...

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