In the frame of the "Ecole de Physique des Houches", a series of courses aimed at selected PhD students and young researchers will be given about fluorescent labels used in advanced fluorescence microscopy: "Fluorescence Markers for Advanced Microscopy: from Photophysics to Biology".
In general, fluorescence microscopy requires labeling the samples with suitable fluorescent markers, either fluorescent proteins, organic fluorophores or nanoparticles.
The markers used in super-resolution microscopy, however, must possess very specific properties. Nanoscopic techniques, in fact, rely fundamentally on the complex photophysical behavior of these markers. There is a global lack of knowledge about how fluorescent markers should be selected, their properties and mechanisms, and the type of artifacts they can create. The objective of the school is to contribute overcoming this lack.