Research scientist, husband and father of two
Molecular biology/biochemistry: cloning, mutagenesis, overexpression, purification...
Macromolecular crystallogenesis and crystallography
Ensemble spectroscopy in solution and crystals (UV/Vis, fluorescence, Raman, lifetime) & single molecule spectroscopy
Conventional optical microscopy and single-molecule localization microscopy (PALM)
I was born in Paris and grew up in a small cute concentric ring-shaped village in the southeastern France before going to middle school at an institution held by Marist Brothers and in a school in Paris and then to high school in the French department Drôme.
I then moved to Grenoble to start Medicine studies and quickly shifted to Biology studies, particularly attracted by biochemistry, molecular biology and structural biology, at the University Joseph Fourier (UJF).
The UJF is named after Joseph Fourier who was appointed Prefect of the department Isère by Napoleon, where Grenoble is located. That's also in Grenoble that he studied and invented his famous series and transform. Although a University was present in Grenoble since as early as 1339, Fourier created the Royal University of Sciences in 1810 where he met his future friend Champollion.
In 2017, the UJF merged with the other universities in Grenoble to form the "Université Grenoble-Alpes" (UGA).
The UGA, third largest French university, is composed of 60 000 students and is well ranked since it is evaluated as being the 163rd research center in the world and the 6th best in France in the research ranking of higher education institutions according to the Scimago Institution Ranking. It is also in the top 150 Universities according to the Shangai Ranking and the 5th in France.
Early in my studies in chemistry/biochemistry, I was attracted by rendering 3D molecules and by the fantastic 3rd generation Synchrotroton standing in Grenoble (ESRF), which is one of the three most powerful synchrotrons in the world. I was lucky enough to start a traineeship there and oriented my studies to structural biology. In parallel of my studies, I carried on being a trainee at the ESRF and trained intensively in X-ray protein crystallography and microspectrophotometry. The team of Dr. Dominique Bourgeois I was working in was particularly interested in studying photosensitive proteins or photoreactive compounds in order to trig events and trap structural intermediate along protein reactions. This is called kinetic crystallography.
Life sciences in Grenoble
I finished my PhD thesis in 2008 and defended it in 2009 at the ESRF, Grenoble. It was entitled "Mechanistic studies of photoactivatable fluorescent proteins: a combined approach by crystallography and spectroscopy".
To read more about my PhD and dowload the full text, go on the dedicated page of this website.
During my thesis, the concept of super-resolution microscopy by photoactivated localization was invented. In this technique, a conventional widefield microscope is used to acquire fluorescence imaging of a biological sample. However, unlike conventional fluorescence microscope, in PALM, the highlighting of proteins of interest is achieved by PTFPs that can be photoactivated, so that only a small fraction of fluorescent molecules can be observed at a time.
The stochastic activation of those individual molecules makes that they are spatially distinguishable and can be precisely localized... with a much better accuracy than that obtained by conventional microscopy where all the molecules emit their fluorescence simultaneously, limiting the achievable optical resolution to ~200-300 nm.
Observing single fluorescent molecules with very sensitive cameras, thus, allows the reconstruction of a composite image that does not suffer from the physical law of far-field diffraction-limited imaging. This type of localization microscopy, invented in 2006 and awarded by the Nobel Prize in Chemistry 2014 permits to obtain ~20-nm resolution images but requires the use of adapted PTFPs.
Using the structural and mechanistic knowledge of PTFPs I could acquire during my PhD, I was extremely lucky to make a postdoctoral period in the laboratory for photochemistry and spectroscopy of Pr. Johan Hofkens. My research was focussed of the engineering and characterization of new types of phototransformable fluorescent proteins and their use in PALM. For this purpose, I used molecular biology techniques, spectroscopy and super-resolution microscopy. This period was very helpful for me to acquire a more applied side to the fundamental knowledge I had about fluorescent proteins. Building optical setups and observing by myself what are the requirements of microscopists for perfoming fluorescent probes completed advantageously my formation of biophysicist.
This lab is located in Flemish Belgium, at the University of Leuven (K.U.Leuven). Founded in 1425, this prestigious University is nowadays well placed in major rankings:
among which the best 100 universities in the world and 2nd in Belgium according to the Top 500 Shangaï academic ranking of world universities.
among the top 100 research centers in the world and the best in Belgium in terms of scientific production according to the Scimago Institution Ranking.
I defended my HDR almost fifteen years after my PhD thesis, considering it as an update of works I have contributed to since this period. It was entitled "Etudes photophysiques et ingénierie des protéines fluorescentes phototransformables" ("Photophysical studies and engineering phototransformable fluorescent proteins")
To read more about HDR and dowload the full text, go on the dedicated page of this website.
At the end of my postdoctoral period, I was recruited as a CNRS researcher to develop super-resolution and conceive future fluorescent markers for advanced microscopy. I had the rare opportunity to come back to Grenoble, at the Institute for Structural Biology in the "Pixel" team led by Dr. Dominique Bourgeois, my former thesis supervisor. Pixel is part of the Integrated Imaging of Stress Response (I2SR) group.
Two years after my arrival, our institute, the IBS, moved to the scientific campus called European Photon and Neutron (EPN) within the so-called "Polygone scientifique" located on the Grenoble peninsula ("presqu'ile) between the river Isère and the mountain stream Drac.
The EPN is one of the three majors campuses of Grenoble and gathers the Institute for Structural Biology (IBS), the European Molecular Biology Laboratory (EMBL), the Institut Laue Langevin (ILL) and the European Synchrotron Radiaton Facility (ESRF) where I've made my PhD thesis.
The moving of the IBS is associated with a major programme called "Grenoble Innovation for Advanced New Technologies" (GIANT) that lead to the foundation of a worldwide ranked innovation campus.
The I2SR group in 2022
From left to right: V. Adam, A. Maity, J. Timmins, F. Lacroix, D. Bourgeois, P. Frachet, P. Tacnet, P. Vauclare, M. Hayek, F. Hans, M. Byrdin, J. Wullfelé, student and J.P. Kleman
The I2SR group in 2021
From left to right: A.S. Banneville, student, A. Mantovanelli, J. Wullfele, P. Frachet, S. De Bonis, P. Vauclare, S. Dufour, F. Lacroix, O. Glushonkov, P. Tacnet, V. Adam, J.P. Kleman, D. Bourgeois, M. Byrdin, F. Hans and J. Timmins
Pixel Team in 2022
From left to right, top row: A. Mantovanelli, V. Adam, D. Bourgeois, M. Byrdin, J. Wullfele
bottom row: O. Glushonkov, P. Frachet, P. Tacnet and A. Maity
Pixel Team in 2017
From left to right: V. Adam, J. Beaudouin, M. Byrdin, D. Bourgeois and D. Thédié
Pixel Team in 2020
From top to down and left to right: V. Adam, D. Bourgeois,
O. Glushonkov, A. Mantovanelli and M. Byrdin
Pixel Team in 2014
From left to right: Top: D. Bourgeois, C. Duan, M. Byrdin, V. Adam -
Bottom: S. Avilov and R. Berardozzi