New publication!

Current Opinion in Chemical Biology (2014) "Phototransformable fluorescent proteins: Future challenges" In fluorescence microscopy, the photophysical properties of the fluorescent markers play a fundamental role. The beauty of phototransformable fluorescent proteins (PTFPs) is that some of these properties can be precisely controlled by light. A wide range of PTFPs have been developed in recent years, including photoactivatable, photoconvertible and photoswitchable fluorescent proteins. These smart labels triggered a plethora of advanced fluorescence methods to scrutinize biological cells or organisms dynamically, quantitatively and with unprecedented resolution. Despite continuous improveme

New publication!

PLoS One (2014) "In cellulo evaluation of phototransformation quantum yields in fluorescent proteins used as markers for single-molecule localization microscopy" Single-molecule localization microscopy of biological samples requires a precise knowledge of the employed fluorescent labels. Photoactivation, photoblinking and photobleaching of phototransformable fluorescent proteins influence the data acquisition and data processing strategies to be used in (Fluorescence) Photoactivation Localization Microscopy ((F)-PALM), notably for reliable molecular counting. As these parameters might depend on the local environment, they should be measured in cellulo in biologically relevant experimental co

Featured Posts
Posts à venir
Tenez-vous à jour...
Recent Posts
Archive
Search By Tags

© 2014-2020 Virgile Adam. All rights reserved

  • LinkedIn
  • Twitter
  • Research Gate
  • Publons
  • Scopus
  • HAL
  • ORCID
  • Google Scholar
  • Mendeley
  • Viadeo
  • Pubfacts